YIQ Visit

On June 27th a group of students participating in the Young Innovator's Quest visited the biotech academy.  We spent some time talking about functional DNA and the future of biotechnology.  We then performed experiments to determine the genus and species of several non-hazardous mushrooms.  

To determine the species of mushroom, we performed PCR reactions on genomic DNA isolated from each mushroom at the biotech academy.

Lane 1, specimen #1; Lane 2, specimen #2; Lane 3, specimen #3; Lane 4, specimen #4; Lane 5, Ladder; Lane 6, specimen #5; Lane 7, specimen #6.

Lane 1, specimen #1; Lane 2, specimen #2; Lane 3, specimen #3; Lane 4, specimen #4; Lane 5, Ladder; Lane 6, specimen #5; Lane 7, specimen #6.

Participants got to choose some of the parameters in the PCR reaction, including the primer concentration, the genomic DNA template concentration, and the PCR program.  The best looking amplicon for each specimen will be sent for sanger sequencing and used to identify the genus and species later this week.

The three PCR programs used for the mushroom amplification.

The three PCR programs used for the mushroom amplification.

Most of the PCR reactions turned out nicely.  The most intense band for each specimen will be sent for sequencing.